Umbilical Cord Blood Stem Cells Bank, Collection of UCSCs during Delivery

Backgroud: Until now, blood that remained in the umbilical cord and placenta after delivery was routinely discarded as garbage. Objective: Evaluation of different factors affecting UCSCs collection and cryopreservation to be used further in transplantation for different therapies. Methods: After approval of Ethics Committee and patient consent, randomly selected 40 Umbilical cord blood samples were drawn from 40 healthy pregnant women during delivery. Mononuclear cell separation was done. CD34+stem cells were obtained using MACS stem cells separator. Cord blood samples were cryopreserved. HLA genotyping was done for screening 8 of the samples by NGS after DNA extraction. Results: UCB stem cells collection was affected by birth weight, sex of new born, volume of fresh blood and length of UC. The percentage of CD34+stem cells viability after thawing was not affected throughout cryopreservation period. HLA genotyping was a good tool by NGS. Conclusions: UCB is a cheap, easily and rich source of hematopoietic stem cells. A chance that is given to man once in life time and particularly at the moment of birth, So SCs are the seeds that deserve to be banked not disposed as garbage. UCSC Collection is non-invasive, painless, and poses no risk. NGS over has a great value in the context of HSCT for cord blood banking which was suggested as a complementary step.