Preparation of Chitosan Material and its Antifungal Activity for Bamboo

The main aim of present study was to prepare chitosan nanoparticle, chitosan-ZnO nanoparticle and chitosan montmorilonite to evaluate their in vitro antifungal activities. The antifungal properties of nanoparticles against phytopathogenic fungi Trametes versicolor and Tyromyces palustris were investigated at various concentrations 0.01 and 0.1 %. Among the various formulations of nanoparticles, chitosan-ZnO nanoparticles were found most effective at 0.1 % concentration and showed 89.5 and 63.0 % growth inhibition of Trametes versicolor and Tyromyces palustris, respectively in in vitro model. At the same concentration, chitosan-ZnO nanoparticles also showed maximum of 87.4 % inhibition rate of spore Trametes versicolor. Chitosan nanoparticles showed the maximum growth inhibitory effects 84.3 % on in vitro mycelial growth of Trametes versicolor at 0.1 % concentration, and 85.8 % growth inhibition of Tyromyces palustris at 0.1 %. From our study it is evident that chitosan material particularly chitosan nanoparticle and chitosan-ZnO nanoparticles have tremendous potential for further field screening towards crop protection.