Molecular Study of Some Virulence Genes in Biotype Diversity of Methicillin Resistance Staphylococcus aureus isolated from Handling Carrier and Bovine Mastitis

Methicillin-resistant Staphylococcus aureus (MRSA) more serious problem in dairy animals suffering from mastitis. In this study collated 130 sample of mastitis milk and 130 from nasal worker. The S. aureus characterized phenotypically by biochemical and API Stph and genotypically by used sua gene specific species. (47.69 %) percentage of isolation in mastitis milk, (82.25 %) biotype C and (4.83 %) biotype B that origin from bovine and (12.90 %) biotype A origin from human, while isolation S. aureus (44.61 %) from nasal carrier, the biotype A recoded 91.37 % and 8.63 % biotype C origin from bovine. The isolates were tested using agar disc diffusion method for oxacillin and cefaxiton and confirmatory mec A gene by PCR (28.12 %) was appeared MRSA from bovine and (35.48 %) from human isolated, all of MRSA have all of virulence genes ctfA gene, Spa gene and Nuc gene for their antimicrobial susceptibility to 10 different antimicrobial drugs. Most of MRSA isolates were found to be multi-drug resistant