Effects of Quercetin and Vitamin D on MCF?7 Cell Proliferation with Real-Time Cell Analysis

The aim of this study was to investigate the effects of Quercetin (Que) and vitamin D (vitD) alone and Que in combination with vitD on proliferation of human breast cancer cells (MCF7) by real-time and continuous monitoring of cell proliferation, and viability. After seeding 200 L (10.000 cells/well) of the cell suspensions into the wells of E-plate 16, cells were treated with Que, vitD and Que+vitD mixtures and monitored every 15 min for 140 hours. Almost a day after seeding, the cells were treated with Que (100-125-150 M) and 140 nM vitD and Que+vitD mixtures. Antiproliferative effect of Que+vitD mixtures started to increase at ~45. hours compared to Que doses and at ~65. hours compared to vitD after treatment. Cell proliferation decreased to 71, 4 % with 150 M mix, 61, 7 % with 125 M mix and 57, 4 % with 100 M mix for 80 hours compared to the control (p=0, 000). When MCF-7 cells were treated with Que, vitD and Que+vitD mixtures, cell viability decreased significantly in time-dependent manner. Exposure of MCF-7 cells to Que+vitD mixture resulted in a clear decrease in viable cells and may be a new and alternative therapeutic approach to breast cancer.