Lactobacillus plantarum modulatory Effect on the Secretion of Interleukin-10,TGF?, and Fibronectin in Macrophages and Skin Dermal Fibroblasts Culture
Journal: International Journal of Science and Research (IJSR) (Vol.6, No. 3)Publication Date: 2017-03-05
Authors : Rita Shintawati; Hernawati; Didik Priyandoko; Sunarjati Sudigdoadi;
Page : 779-784
Keywords : Lplantarum; IL10; TGF; fibronectin; imunomodulator;
Abstract
Impaired wound healing in diabetic patients is associated with increased pro-inflammatory cytokine TNF? and IL1?, as well as the reduction of inflammatory cytokines IL10 and TGF?. Lactobacillus plantarum is known to modulate the immune response towards the regulatory cytokine that increases anti-inflammatory cytokines. The purpose of this study is to determine the effect of L. plantarum on the secretion of IL10, TGF?, and fibronectin of macrophages and dermal fibroblasts. Research using cell culture of peritoneal macrophages and dermal fibroblasts of Wistar rats, cultivated in RPMI + 10% FCS, and stored in a 5% CO2 incubator at 37?C. S. aureus atcc 25923 in Mueller Hinton and L. plantarum atcc 8014 in MRS were harvested at mid-log phase and then the suspension was made at doses of 106, 107, 108 cells/ mL and subsequently heat-killed. Macrophage cultures were exposed to S. aureus for 6 hours washed and then treated by combination of S. aureus-L. plantarum (SALP) and L. plantarum for 24 hours. Next the supernatant was separated, added into the culture of fibroblast cells for 24 hours prior to measurement of IL10, TGF?, and fibronectin levels by ELISA. Analysis using ANOVA and Tukey's tests with ? = 5% shows the results where overall exposure of L. plantarum along with increasing doses had increased the levels of TGF? macrophages and fibronectin fibroblasts, meanwhile SALP exposure increased the lowest level of fibronectin. Increased level of IL10 fibroblasts was reached only on the highest dose of LP exposure. Thus, it was concluded that L. plantarum increased the levels of TGF? and fibronectin (p less than0.000).
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