Activity of Glutathione-s-transferase in Trigonella foenum-graceum Whole Seed Powder Treated Diabetic Murine Model System

Humans have highly developed antioxidant systems, which works well within the body and can easily fight with free radicals that causing oxidative stress. The increased oxidative stress can cause initial cell damage in type I diabetes, or impaired insulin production, release or function in type II diabetes. Glutathione-S-transferases (GSTs) are a family of Phase II detoxification enzymes that catalyse the conjugation of glutathione (GSH) to a wide variety of endogenous and exogenous electrophilic compounds. GST catalyzes attachment of the thiol of glutathione to electrophiles, an important condition affecting GST expression is oxidative stress. Trigonella foenum-graceum is one of the oldest cultivated medicinal plants identified in written history, and many studies showed that the its seeds acquire anti-oxidant properties. Our study evaluated that effect of different doses (5, 10 and 15 mg/kg) of Trigonella foenum-graceum whole seed powder show magnitude of elevation in GST activity of both liver and kidney. In liver when compared with normal control the specific activity of GST is significantly reduced by 0.45 folds in diabetic control group. When compared to normal control low dose treated group show significant decrease of 0.67 folds, medium dose group show decrease of only 0.72 folds but not significant while high dose treated group show minimum reduction of 0.87 folds. Similarly the specific activity of GST is significantly reduced by 0.40 folds in diabetic control group as compared to normal control. Low dose treated group shows significant reduction by 0.68 folds and in medium dose treated group decrease of 0.79 folds was observed. High dose treated group animals shows minimum reduction by 0.81 folds but both medium and high dose had no significant change.