Antimicrobial Screening of Scoparia dulcis and Eclipta alba Plant Extract

Medicinal plants are an amusing reservoir of therapeutic agent and nowadays pathogenic microorganisms are becoming resistant and altering themselves into multidrug safe strains because of use of aimless antibiotic numerous medication. Because of numerous microbes resistant lot of antibiotics are not much effective in the treatment of infection. To fight against these human pathogens there is a need of introduction of new antibiotics. In this study, antimicrobial activity of all extracts was observed by Agar well diffusion method and 96 well plate-microdilution method for their minimum inhibitory concentration. In Scoparia dulcis plant chloroform, ethyl acetate, acetone and methanol extract showed good zone of inhibition against B. subtilis, E. coli, Strep. group B, S. pyogene while Eclipta alba showed good zone of inhibition for methanol against B. subtilis, chloroform against C. albicans, chloroform, acetone and methanol against S. pyogene and hexane against Streptococcus Group B. E. coli showed sensitivity against all five solvents (hexane, ethyl acetate, chloroform, acetone and methanol extract). MIC concentration indicated lowest concentration in hexane, ethyl acetate, chloroform and methanol extract of Scoparia dulcis against E. coli, ethyl acetic, chloroform and methanol against Strep. group B, methanol and methanol extract against S. pyogene, ethyl acetate against C. albicans, and Methanol against S. aureus demonstrated the indicated important significance of antibacterial activity with alternate extracts whereas Eclipta alba showed that hexane, chloroform, acetone and methanol extract against E. coli, chloroform against C. albicans, ethyl acetate, chloroform, acetone, and methanol extract against S. pyogene and methanol against B. subtilis are significant for antimicrobial activity. The current research confirms that both the plants are sensitive towards the pathogenic microorganism.