Content of Ajmalicine on Cultured Callus Catharanthus roseus (L.) G. Don with Tryptophan Treatment

Catharanthus roseus (L. ) G. Don is cultivated as an ornamental plant and also has a high economic value because it contains terpenoid indole alkaloids such as ajmalicine that have pharmacological benefits. This research was conducted to investigate the content of ajmalicine in callus culture that was given tryptophan treatment with concentrations of 50 mg/L, 100 mg/L, 150 mg/L, 200 mg/L and 250 mg/L. Identification and analysis of ajmalicine using HPLC Shimpak VP-ODS C18 150 x 4.6 mm at a wavelength of 254 nm. The results showed that the ajmalicine content was lower after the tryptophan treatment, where the minimum ajmalicine content of the tryptophan 200 mg/L, ie 0.346 g/g dw. Ajmalicine content of the treatment of 250 mg / L tryptophan, is the highest content in all treatments, ie 2.686 g/g bk. So it can be concluded that the treatment of tryptophan in callus culture Catharanthus roseus can decrease the content of ajmalicine.