Anti-implantation and Antiestrogenic Activity of Ethyl Acetate Extract of Chromatographic Fractions of Eugenia Jambolana Seeds in Female Albino Rats

Plants are a valuable source of new natural products. Ancient literature mentions the use of a number of plants preparations for fertility regulation. In the present study, the ethyl acetate extract of Eugenia jambolana seeds which showed promising antiimplantation and antiestrogenic activity in female albino rats was examined for the isolation of its active fractions. Two fractions (I& II) were obtained using Thin Layer Chromatography (TLC) of the extract. Both fractions were subjected for testing their antiimplantation and antiestrogenic activity in rats. The data revealed that the fraction I of ethyl acetate extract treatment at both dose levels decreased the number of implants compared to control group. The fraction I exhibited a strong antiestrogenic activity, when administered alone. It also inhibits the estrogen induced gain in the uterine weight when administered along with ethinyl estradiol. Histological studies of the uterus were carried out to confirm this activity. These findings suggest that a chromatographic fraction I of ethyl acetate extract of E. Jambolana seed might be used as a contraceptive in the females.