Flow Injection Turbidimetric Determination of Vitamin B1using LEDs as a Source of Irradiation and Two Solar Cells as an Energy Transducer

A sensitive and simple method has been used for the determination of vitamin B1 (thiamine chloride) in pure form and pharmaceutical tablets by flow injection turbidimetry. The method is based on formation of ion pair compound between thiamin chloride and ammonium molybdate in an aqueous medium to obtain a white precipitate complex, using homemadeAyah-6SX1-ST-2D solar cell CFI Analyzer. Optimum parameters have been studied to increase the sensitivity for developed method. The linear range for the calibration graph was 0.1-2 mMol. L-1 vitamin B1 (VB1) and LOD was 1.579g/sample with correlation coefficient ( r) was 0.9708, RSD % was lower than 0.5 % the repeatability (n=8, 6) for the determination of VB1 at concentration (0.9, 1.5) mMol. L-1 respectively. The developed method was applied successfully for the determination of VB1 in pharmaceutical tablets. A comparison was made between two methods developed method and the classical method UV spectrophotometry at max=246 nm, by using the standard addition method via the use paired t-test. It showed that there was no significant difference between the developed method and the classical method for determination vitamin B1at 95 % confidence level.