Detection KIT D 816 Mutation and its Association with Outcome of Sudanese Acute Myeloid Leukaemia Patients at Khartoum State

Background Acute Myeloid Leukemia (AML) is a fatal disease and have increasing prevalence in Sudan and occurs at all ages from the neonatal period to old age. It has multiple factors caused AML. One of the important these reasons the cytogenetic abnormalities what may affect the outcome in patients with AML include FLT3, KIT, CEBPA, BAALC, ERG, MLL, and NPM1. This study conducted to detection KITD 816 mutation and its association with outcome of Sudanese Acute Myeloid Leukaemia's patients at Khartoum state under chemotherapy treatment within 30 diagnosed AML patients in different types of FAB classifications were selected 14 (46%) male and 16 (53%) female. The age was range from (5-70 years) during the period from May 2015 to May 2017 at Radio-Isotopcenter Khartoum, Omdurman Military Hospital, GafarIbn auf paediatric hospital. Result this study showed significant association between frequency of KIT D816 mutations and incidence of AML disease (p. value=0.00) the percentage (76.6%) of patients positive for KIT D 816 mutation and (23.3%) of patients negative for KIT D 816 mutation. Also There was showed insignificant association between KITD816 mutation and AML patient's outcome under chemotherapy treatment as general (p. value=0.666) but there is insignificant association between KIT D 816 mutation and AML patient's outcome with (p. value=0.666) but there was significant association between adverse patient's outcome with KIT D 816 mutation if combination with some types of AML (M0, M4 and M5) comparing with control group with (p. value= 0.008). Also there was significant increased frequency in male by (43.3%) more than female (33.3%) with (P. value=0.05) and there was insignificant association between KIT D816 mutation and age of patients (P. value=0.55). Conclusion KIT D 816 mutation had significant association with AML disease at different types and especially affected male more than female, generally it had insignificant affect in outcome of AML.