Isolation, Identification and Anti-Diabetic Activity of Isorhamnetin-3-O-?-L-(3??-E-p-coumaroyl)-Rhamnoside from Solanum torvum

To isolate a flavonoid glycoside from flowers of Solanum torvum using chromatography separation techniques, spectral characterization and its anti-diabetic activity. The structure of the isolated compound was purified, analyzed and characterized by chemical tests, HPLC and spectroscopic methods such as UV, IR, 1H-NMR and 13C-NMR. HPLC analysis exhibited the presence of a flavonol glycoside and its identified peak with the retention time of 26.8 min and showed two major peaks at 340 nm (band-I) and 260 nm (band-II). The flowers of Solanum torvum have been found to contain isorhamnetin-3-O--L- (3-E-p-coumaroyl) -rhamnoside. The blood sugar level was measured by digital display glucometer. The highest activity of Solanum torvum flower extract in this experiment was observed at the dose of 200 mg / kg of isolated compound while the reference drug glibenclamide (10 mg/kg) had a greater activity when associated with Solanum torvum flower extract.