Novel Tumor Markers of Breast Cancer Patients

Alibha Rawat1, N. Ganesh1* Objective The objective of the present study is to look for some Novel Tumor Markers for the diagnosis of Breast Cancer and screening females having high risk of developing Breast Cancer by exploring the Anthropometric, Cytogenetic and Biochemical Technique. Method The study consists of three parameters for which informed consent were taken from each individual. First for the Anthropometric Analysis, angle of triradius (atd) angle of around 55 Breast Cancer patients (age group 18-70 years) along with age matched 55 Healthy Control females were taken ( Harold Cummins, 1960). For the Cytogenetic Analysis, peripheral blood of the same group of individuals were subjected to lymphocyte culture by standard protocol of Moorehead et. al. , (1964) and chromosomal aberrations were observed by karyotype preparation. For the Biochemical Analysis, plasma samples of both the groups were studied by running them in cellulose acetate membrane in an Electrophoretic chamber (Robyt, John F, 1990) and any altered plasma protein patterns that were specific for Breast Cancer patients were observed. Result In the Anthropometric Analysis using dermatoglyphic tool atd angle, Breast Cancer patients were found to be having wider angles (greater than42) than their healthy counterparts. In the Cytogenetic Analysis, Breast Cancer patients were showing a much higher frequency of numerical as well as structural aberrations than the healthy females and in the Biochemical Analysis, Breast Cancer patients were found to be having a raised beta globulin proteins while healthy females showed a normal range. In addition, most breast cancer females showed a gamma globulin pattern of polyclonal origin. Conclusion These parameters in combination have a potential to be used as Tumor Markers for the diagnosis of breast cancer and these are not only cost effective but also non-invasive which makes them more accessible especially for screening females at high risk of developing Breast Cancer.