HPLC Analysis of Flavonoids in Acanthophora Specifera (Red Seaweed) Collected from Gulf of Mannar, Tamilnadu, India

Acanthophora Specifera is a species of marine red seaweed and in the family Rhodomelaceae. They were freshly collected from Mandapam Coastal Area, Rameswaram Tamilnadu, India and rinsed in seawater and packed in aseptic bags for further proceedings to laboratory. Seaweeds are potential renewable resources in the marine environment. It has been used as antioxidant and antimutagen. Methanol extract was prepared for further analysis. HPLC analysis of Acanthophora Specifera was carried out with Chromatographic system (Shimadzu Class-VPV6.14SP2, Japan) consist of autosampler with 20l fixed loop and an UV-Visible detector. This investigation was carried out to determine the possible flavonoids components from Acanthophora specifera by HPLC analysis. In the present study total six flavonoidal compounds were detected. The percentages of major flavonoids contents were chlorogenic acid (69.64 %), Caffeic acid (12.86 %), Vitexin-rahmnose (5.20 %), Querecetin (0.59 %) and Catechol (1.41 %). These compounds have main physiological role and pharmaceutical effect on the Acanthophora specifera and also same role on the animal and human physiology.