Estimation of Eugenol Derived Chemical Compound Activity on Intestine Tissue and Some Enzymes of Infected Mice

This research aimed to determine the effect of synthesized chemical derivative of plant extracted and purified eugenol on the intestine of the albino mice infected by Escherichia coli in addition to its effect on liver enzyme ALP and creatinine Phenolic compound eugenol was isolated chemically in laboratory , its purity was confirmed by HPLC technology in percent of 90%- 100%. The purified eugenol was chemically derived producing three new synthesized derivatives derived eugenol compound 1 (D. E. C.1) (methoxy-1- bromo prop-phenol acetate) Experimental animal were used to detect the inhibitory effect of eugenol derivative on pathogenic bacteria E-coli was by injection in vivo. Experiment design included use of 10 mice divided in 5 groups the target organ was (intestine) which was isolated from dissected mice, blood test and biochemical test (Creatinine, Liver enzyme ALP) were performed. Results showed that mice treated twice with D. E. C.1 10% for ALP and creatinine in mice treated twice with D. E. C.1 at 10% was nearly normal value as in the control group. Histological results showed changes in the intestine ranging from hemorrhage, , degeneration and lymphocyte accumulation in infected non- treated mice while the treated mice with D. E. C.1 derivative's showed gradual regeneration after one week.