Microbial Production of Protease: Isolation and Optimization

The microbial production of protease is the need of rapidly growing industrial field. So due to its wide utility protease production becomes the centre of attraction for commercial market. The present study emphasized on microbial production and optimization of protease enzyme. The potential protease production depends on effective strain selection, for this purpose we took different samples of stagnant water from different locations. Then we isolated the single colony of protease producing microorganism from it, through repeated sub- culturing. The presence of protease enzyme in the microbial culture was checked by skimmed milk assay followed by protease assay. Then the effective strain is selected for the optimization. The protease enzyme was optimized in various carbon sources such as glucose, starch, molasses, and glycerol for potential protease production.