Effect of Precursor beta-phenylalanine on Production of Flavonoids of Maytenus emarginata in vitro

Medicinally important Maytenus emarginata is an ever green tree of desert useful against various diseases. Flavonoids have been reported in its plant parts. In present study unorganized tissue raised on MS medium supplemented with1.5 mg/L BAP +1 mg/L NAA, harvested at maximum GI, were analysed for qualitative and quantitative estimation of flavonoids by Subramanian and Nagarajan (1969) method. These tissues were fed with different concentration of dl -phenylalanine (25, 50, 75, 100 mg/100ml) to observe the effect on GI and quantitative production of flavonoids. Maximum GI and amount of flavonoids (leutiolin, kaempferol, quercetin and total flavonoid) was estimated in eight weeks old tissue fed with 75 mg/100ml phenylalanine.