Genetic Transformation&Gus Gene Expression in Piper longum

Piper longum is a medicinal plant of great importance. It has antioxidant, anticancerous& antibacterial activity. Genetic transformation of Piper longum can improve its existing properties for the betterment of mankind. Gus gene expression was observed in in vitro grown callus tissue of Piper longum when it was transformed using Agrobacterium tumefaciens Agrobacterium strain containing plasmid pBECK2000 carrying GUS gene and kanamycin gene is used. The Agrobacterium strain at concentration 600nm of 0.538 OD and callus of Piper longum was Co-cultivated at 28C for 2-3 days incubation, at different acetosyringone concentration (50-200M) infection time varies from 5-10 min. Genomic DNA was isolated from the infected callus and Molecular analysis for Gus gene expression in plant was done by Polymerase chain reaction which shows stable transformation. In this paper Piper longum is transformed to raise transgenic plants of Piper longum with improved medicinal properties