In vitro propagation of Cleome gynandra L. through direct organogenesis

An efficient protocol was developed for in vitro regeneration of Cleome gynandra L. is a herb belonging to the family Cleomaceae. In the present study, shoot tip explants of Cleome gynandra were cultured on MS medium supplemented with different concentrations of BAP and Kin (5-25 M/l) alone or in combination with IAA (2 10 M/l) for multiple shoot bud induction. The highest frequency (100 %) of shoot bud induction and maximum number of shoot buds (26.40.48) was noticed on 10M BAP with mean shoot length of 8.2 cm. The shoot proliferation was further improved by combination of BAP+IAA. The maximum number of shoots (35.8 1.42) was developed on MS media containing 10 M/l BAP in combination with 2 M/l IAA. The regenerative shoot buds were transferred to half strength MS medium fortified with different concentrations of IBA and NAA (2-10M/l) for root induction. The maximum numbers of roots (17.21.95) were obtained from 4M NAA with mean root length of 3.6 cm. In vitro regenerative plantlets were successfully acclimatized in red soil, farmyard manure and sand (2: 1: 1). Subsequently, the plants were successfully established in the field.