Pathogenresistant and Insertion of Monellin in Sugarcane by Fusion Protein and Particle Bombardment (Sugarcane for Diabetic Patients)

Sugarcane is tall perennial grass of the genus Sacharum. Sugarcane has a sucrose content of 10-18 % and a fiber content of 10-15 % at harvest. Sugarcane is the 2nd largest cash crop of Pakistan (Naz. , 2003). Sugar cane commercially very important crop. Sugarcane is a primary raw material for production of sugar ( M. Irfan Shaukat). Sugarcane has been chosen as a crop for improvement because of its potential for high biomass production and therefore application for the production of bioenergy and bio materials. The prevalance of the pathogens constitutes on abnormal condition, affecting plant growth and impairing important physiological process. To increase sugar contents of sugarcane monellin insert because monellin is 3000 time sweeter than sucrose and 1000, 000 more sweeter than sugar on molar basis it is suitable for diabetes. (Rosalind kim et all. , 1992). For better production of sugarcan it should remove the pathogens and make it pathogenic resistance and for this purpose chitinase use. Chitinase are enzymes that catalyze the hydrolysis of - 1, 4 N-acetyl glucosamine linkage present in chitin. As chitin is a major component of fungal cell walls and is absent in plants, chitinase play a role in plant defense against pathogens (Schlumbaum el al. , 1986). For this purpose construct form by using 35s promotor and in construct monellin, chitinase and promoter use and when construct complete it can transfer insugarcane by particle bombardment. When construct will be inserting then reporter gene show color then we observed gene has been express in stem of sugarcane