Biochemical Screening of persea macrantha (NEES) Leaf extracts for its Antioxidant and Cytoprotective Properties

Plants are prosperous source of antioxidants. In the current investigation aqueous and methanolic extracts of persea macrantha were tested for total phenolics, radical scavenging activity, reducing power, inhibition of lipid per oxidation, DNA protection and cytoprotective effects. The aqueous and methanolic extracts exhibited 5.57mg and 8.80mg of total phenols/g of leaf. In addition to this 60 % and 72 % of free radical scavenging activity were seen /100l of the extracts. Methanolic extracts exhibited extra reducing power activity as well as DNA fortification property when compared to aqueous extract. In RBC cytoprotection studies aqueous and methanolic extract showed IC50 value of 1.57 and 1.05 mg of phenols/ml. In case of lipid peroxidation inhibition, methanolic extract exhibited IC50 value 8.88 g of phenol/ml and aqueous extract exhibited 17.89 IC50 value.