Antimicrobial Activity&Anti Oxidant Activity of Extracellularly Synthesized Silver Nanoparticles from Marine Derived Aeromonas salmonicida

The process of development of reliable and eco-friendly metallic nanoparticles is an important step in the field of nanotechnology. Novel applications of nanoparticles and nanomaterials are rapidly emerging day by day. To achieve this, use of natural sources like biological systems become essential. In the present investigation, Silver Nanoparticles are biosynthesized extracellularly using Aeromonas salmonicida, isolated from marine sediment samples of Tuticorin coast, Tamil Nadu, India. The biosynthesized silver nanoparticles were initially confirmed by visual observation with the appearance of yellowish brown solution in the reaction mixture. In UV-visible spectroscopy, the spectra showed a strong surface Plasmon resonance centered at 420 nm. The silver nanoparticles were found to be face centered cubical in shape with variable size ranging from 20 to 80 nm as obtained by SEM analysis. X ray diffraction (XRD) data illustrated the crystalline nature of silver nanoparticles. The possible biomolecules such as amines and polyphenols may responsible for reduction of silver ions was identified through FTIR Spectroscopy. The antimicrobial activity of synthesized nanoparticles was affirmed against bacterial pathogens Bacillus subtilis, Enterobacter aerogenes, Escherichia coli, Klebsiella oxytoca, Staphylococcus aureus and Streptococcus pyogenes. Further the anti oxidant activity of silver nanoparticles was analyzed by DPPH assay.