Nutritional Parameters for Growth Profile Study of Protease Producing Halotolerant Bacteria from Mining Site of Kutch, Gujarat

Extensive research on proteases from various sources is thrust area of research as they form a very important part of industrial enzymes. Bacillus sp. producing high yield of protease was isolated from waste water (pH 2.0) of Panandhro Lignite Mine of Kutch, Gujarat. This organism was studied for nutritional parameters viz. as NaCl concentration, substrates and carbon sources for its protease activity. Nutrient broth was used as fermentation medium. Protease activity was measured by Folin Phenol method. Maximum protease activity (1370 � 0.7 U/ml) was obtained using nutrient broth (pH 7.2) supplemented with 1% w/v Lactose (carbon source), 2% w/v peptone (substrate) when incubated at 37 �C for 24 hours. In addition these protease is halo-tolerant (tolerates 4 % w/v NaCl concentration) which proves its utility in saline areas.