An Investigation about the Effect of UV-Ray on Activity of Laccase and Manganese Peroxidase in Pleurotus Florida Shell Fungus
Journal: International Journal of Science and Research (IJSR) (Vol.4, No. 3)Publication Date: 2015-03-05
Authors : Sara Saadatmand; Fatemeh Sohrabi;
Page : 1419-1425
Keywords : Pleurotus Florida; Laccase; Manganese Peroxidase; UV-Ray; Shell Fungus; Plant Physiology; Biology;
Abstract
Lignin is the second most abundant compound on the earth and the most resistant one against decomposition. Fungi are able to produce Ligninases and to secrete them to their surroundings. Among them, white rot Basidiomycetes are very important. Pleurotus species are one of the Basidiomycetes. In the current research, the effect of UV-B ray on activity of Laccase and Manganese Peroxidase in Pleurotus Florida shell fungus is studied. In this regard, the Mycelium of Pleurotus Florida fungus was cultured in a liquid environment containing mineral elements and Guaiacol, during a ten days incubation period and after fifth days from the start of culturing UV-ray was applied to Pleurotus Florida fungus in four periods of time (0, 1, 2, 3, and 4 hours). The obtained results show that applying UV-ray in 1, 2, and 3 hours periods have not considerably changed wet weight and dry weight of Mycelium but during 4 hours of UV-ray applying, these weights are significantly reduced. At the other hand, applying UV-ray to Mycelium of fungus causes to significant increase in activities of Laccase and Manganese Peroxidase.
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