Genetic Variation and Phylogeny Assessment of Aciagrion Occidentale (Odonata: Coenagrionidae) Using Mitochondrial Cytochrome Oxidase Subunit I Gene

Aciagrion occidentale is a migratory species widely distributed in montane and submontane areas in open grass besides weedy ponds and herbage. Here we used the COI barcode locus (522bp) to clarify the specific taxonomic status of Aciagrion occidentale. Molecular data suggests that this species is having 99 % sequence similarity to Aciagrion borneense found in Netherland and confirmed that both are sharing same genus. The N-J tree constructed with BLASTn result depicted that Aciagrion occidentale is phylogenetically very close to damselflies. Anisopterans and Zygopterans are sharing some common characters and the results confirm that Zygoptera is a paraphyletic group derived from the monophyletic groups of Anisoptera and Anisozygoptera.