In Silico and in Vitro Reverse Genetics of yebV of Escherchia Coli

Out of 4300 protein coding genes, 850 still have no attributed function. yebV is one such gene that is highly conserved in bacterial domain and not been characterized yet. It may be due to gene-s varying expression levels in different phases or its small size or its non essentiality character. In this paper, we have studied different physiological nature of cell mutated in yebV gene, also tried to model this protein in insilico methods.