Callus culture of Spilanthes paniculata (DC) Jansen- An Aromatic Medicinal plant

Spilanthes paniculata (DC) Jansen is an important medicinal plant with rich source of therapeutic and medicinal constituents of family Asteraceae. Callus culture of Spilanthes paniculata (DC) Jansen was established in Murashige and Skoog-s media augmented with various concentration/combination of growth regulators by using leaf, nodal and internodal explants. Maximum callogenic response i. e.84.62 % from internodal explants was shown at BAP 1.75 mg/l, 74 % was shown at BAP 1.5 mg/l from nodal explants and 75 % from leaf explants at 2, 4-D 1 mg/l.