Nanobacteria ? CNPs the Possibility of Bacterial Association of the Dental Calcifications

Nanobacteria - CNPs the possibility of bacterial association of the dental calcifications Aleksova Pavlina University Dental Clinical Center -St. Panteleimon-- Deparment of Restorative dentistry and Endodontics, Faculty of Dentistry, University -Ss. Cyril and Methodius-, Skopje, R. of Macedonia. Correspondence Aleksova Pavlina Department of Restorative dentistry and Endodontics, Faculty of Dentistry, University-St. Cyril and Methodius-, Skopje, Republic of Macedonia Tel. +389 070 260 308 E-mail pavlinaaleksova@yahoo. com Introduction The discovery of nanobacteria, also referred to as calcifying nanoparticles (CNPs), led to novel theories for pathological calcification. Materials and Methods The present study was analyzed seven dental pulp stones of the molars from a patient, with kidney stones, were detected and leached at University Dental Clinical Center -St. Panteleimon-- Deparment of Restorative dentistry and Endodontics, Faculty of Dentistry, University -Ss. Cyril and Methodius- in Skopje. Control group was samples hydroxyapatite. We used a method of Infrared Spectroscopy, known as FTIR-spectrometry and a method of Atomic-absorption spectroscopy in laboratory at the Faculty of Chemistry, University -Ss. Cyril and Methodius- in Skopje. Results Spectrograms in absorbance were mainly made up of albumin. The intensive line in the spectra in transmittance in the high frequency part, as well as the lines in the area around 1600 to 1300 cm-1 show the presence of albumin, and the intensive line in the area around 1000cm-1 and the doublet of lines around 600 cm-1 are the result of the vibration of phosphate ions present in the stones as carbonate apatite (Calcium Phosphate Carbonate). With the method of atom-absorbing spectroscopy, we got the following results in denticles, in all 7 samples, the value of Ca expressed in mg/kg was 18.501, and 18.502 in %. The value of P in mg/kg was 11.001, and 17.602 in %. Control hydroxyapatite was correctly identified in the test. Discussion Generally, the apatite formation required elevated Pi and/or Ca2+ levels and AP activity. In the nanobacterial model, apatite was formed at [Ca] 1.8 mM and [Pi] 0.9 mM or less. Values Ca/P ratio in dental pulp stones of this study, is such with values in the nanobacterial model. Infrared spectroscopy showing the distinct phosphate absorption signature at 1, 000 to 1, 200 cm21 seen in all four spectra, with inorganic apatite, bone, kidney stones, and nanobacteria (NB). Conclusions With this study we confirm that Nanobacteria or CNPs can be the possibility of bacterial association of the dental calcifications.