In Vitro Plant Regeneration of Luffa acutangula Roxb. Var amara Lin.: An Important Medicinal Plant

Luffa acutangula Lin. Var. amara (Roxb) is an important wild medicinal plant belongs to the family Cucurbitaceae. All parts of the plant are used to cure many diseases, especially in crystalline bitter compound with cucurbitacin B and Amarinin. The whole plant is used to cure many diseases such as Anti-Cancer, Anti-Diabetic, Anti-Jaundice and many ailments. The study was conducted to analyze the effect of plant growth regulators on callogenesis, direct and indirect organogenesis of Luffa amara. Callus culture was induced from leaf and stem explants of Luffa amara in a different hormonal (TDZ + 2, 4 - D, IAA + IBA) combination of MS medium at various concentrations. The best callogenesis response was observed in 2, 4 - D + TDZ - 2.0 mg/L. The nature of callus was produced in hard, green and compact. The good response of callus to produced more number of shoot formation through indirect organogenesis at different concentrations (BAP). Best response of shoot formation was observed in 2.5 mg/L of BAP. The shoots were transferred in both full and half strength MS medium supplemented with Auxin at various concentration (IAA + IBA).