Effect of BAP and NAA on In vitro Shoot Establishment and Proliferation of Banana (Musa paradisiaca) Cv.Grand naine

The study aimed to investigate the effect of different concentration of BAP (6-benzylaminopurine) (0.0, 2.5, 5.0, 7.5 and 10.0 mg/l), NAA (-naphthalene acetic acid) (0.0, 0.5, 1.0, 1.5 and 2.0 mg/l) on shoot establishment and multiplication of banana Cv. Grand Naine. Among the different concentrations used 5mg/l BAP showed shortest days to shoot induction (11 days). In NAA levels, zero concentration was observed as fewer days (13.5). As interaction concerned 5 mg/l BAP + 0.5 mg/l NAA found shortest days to induce which was recorded as 10 days. In shoot proliferation, 5 mg/l BAP observed highest shoot number (2.0 and 2.6 shoots at 15 and 30 DAI respectively).0.5 mg/l NAA showed maximum number of shoots in 15 DAI and 1.0 mg/l NAA at 30 DAI. The interaction of 5.0 mg/l BAP + Controlled NAA showed 2.67 and 3.0 shoots at 15 and 30 DAI respectively. The longest shoot (4.2 and 4.9 cm) at 15 and 30 DAI respectively was produced by the treatment of 5 mg/l BAP. With 1.5 mg/l NAA level 3.0 and 3.7 cm shoot length observed in both 15 and 30 DAI respectively. At interaction 5 mg/l BAP +2.0 mg/l NAA was shown long length of shoot (5.07 and 5.83 cm) in 15 and 30 DAI, respectively. The maximum number of leaves (3.4 and 4.4 during 15 DAI and 30 DAI, respectively in media supplemented 5 mg/l BAP) (2.6 and 3.4 at 15 and 30 DAI respectively in 1.5 mg/l NAA) and (4.0 and 5.3 at 15 and 30 DAI, respectively in interaction of 5.0 mg/l BAP + 2.0 mg/l NAA). The treatment of 5 mg/l BAP was produced longest leaves 3.4 and 3.9 cm at 15 and 30 DAI respectively, while NAA level of 2.0 mg/l produced longest leaves which were 2.5 and 3.0 cm at 15 and 30 DAI, respectively. In interaction 5 mg/l BAP + 2.0 mg/l IAA developed 4.43 and 5.27 cm at 15 and 30 DAI respectively.