Development and Validation of the RP-HPLC Method for the Analysis of Chloropyrifos
Journal: International Journal of Science and Research (IJSR) (Vol.4, No. 5)Publication Date: 2015-05-05
Authors : K. Vinod Kumar; B. Ramachandra; N.V.S.Naidu;
Page : 1081-1085
Keywords : Chloropyrifos; RP-HPLC Method development; Validation;
Abstract
A simple, selective, precise and accurate High Performance liquid Chromatographic method for the analysis of Chloropyrifos in its formulations was developed and validated in the present study. The mobile phase consist a mixture of Acetonitrile and Potassium Dihydrogen Orthophosphate in the proportion 7030 and adjust the pH to 6.8. This was found to give sharp peak of Chloropyrifos at a retention time of 6.780 min. HPLC analysis of Chloropyrifos was carried out at a wave length of 289 nm with a flow rate of 1.0L/ min. the linear regression analysis data for the calibration curve showed a good linear relationship with a regression coefficient 0.999 in the concentration range of 12mg to 72mg. The linear regression equation was y = 63577x + 14260. The developed method was employed with a high degree of precision and accuracy for the analysis of Chloropyrifos. The method was validated for accuracy, precision, robustness, ruggedness, specificity. The precision, accuracy, sensitivity, short retention time and composition of the mobile phase indicated that this method is better for the quantification of Chloropyrifos.
Other Latest Articles
- Anatomical Variants of Circle of Willisin South Indian Population: A Study by Using Magnetic Resonance Angiography
- Denoising of Images Corrupted By Mixed Noise Using Improved WESNR Method
- Power Flow Analysis of Long Transmission Line Using SVC, SSSC and UPFC Integrated with Wind Power
- Review On design and Implementation of Enhanced Security in Multicloud Storage System Using Distributed File System
- Efficiency Improvement Opportunity in Boiler Without Changing GCV of the Coal
Last modified: 2021-06-30 21:46:31