Study the Prevalence and Risk Factors of Metallo-Betalactamase Producing Pseudomonas aeruginosa from Teriary Care Centre

Background- P. aeruginosa is an important hospital pathogen and usually difficult to treat as it is resistant to most commonly used antibiotics. When this organism acquires the gene Metallo-betalactamase (MBL) production, the treatment options become even more limited. It is thus necessary to identify such organisms at the earliest. This is important to prevent the spread of such pathogens in the hospital environment resulting in resistant hospital acquired infections. However, currently CLSI does not recommended any guidelines for a quick phenotypic identification of Metallo-betalactamase producing organisms.9So, the present study was done to identify the prevalence of Metallo-betalactamase producing P. aeruginosa causing infections in a large tertiary care centre and to identify the risk factors associated with these highly resistant infections in a health care setting. Material and method- In this study we studied prevalence, following standared methods of isolation and identification techniques of these bacteria from all clinical samples such as Pus, Sputum, Urine, Blood, CSF etc. For detection of Metallobetalactamase production we were use different phenotypic methods i. e Modified Hodge Test, Im-EDTA disc diffusion method, I m-2MPA double disc synergy test and E test strips. Results- A total of 770 clinical isolates of P. aeruginosa were obtained from various clinical samples over a period of two years.265 ( 34.4 %) of these isolates were imipenem resistant. The prevalence of MBL producing Ps. aeruginosa in the present study was computed to be 32.9 %. Conclusion- To overcome this problem, regular monitoring of the incidence of such organisms in various critical areas of hospital is important. So that isolation and barrier nursing of these patients would prevent the spread of drug resistance in the hospital.