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Immobilization of Little Millet (Panicum sumatrense) ?-amylase

Journal: International Journal of Science and Research (IJSR) (Vol.4, No. 6)

Publication Date:

Authors : ; ;

Page : 150-154

Keywords : amylase; little millet; immobilized; calcium alginate; entrapment;

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Abstract

Alpha amylase (1, 4--D-glucan glucanohydrolase, EC 3.2.1.1) from germinating little millet seeds purified to homogeneity was entrapped in 2 % calcium alginate beads and gave immobilization yield of 65 %. It was observed that entrapment of -amylase was not affected by pH. Temperature optima of immobilized enzyme was higher compared to free enzyme. The enzyme on immobilization showed an apparent Km of 2.810-3g/ml. Immobilized enzyme showed the highest operational stability for upto 7 reuses by retaining 72.5 % of residual activity.

Last modified: 2021-06-30 21:49:27