Rp-Hplc Method Development and Validation for the Analysis of Pharmaceutical Drugs- 60 % Sodium Lactate
Journal: International Journal of Science and Research (IJSR) (Vol.4, No. 6)Publication Date: 2015-06-05
Authors : Bhaskarrav Makwana; V. G. Patel; Vipalben Patel; Ami Patel;
Page : 1532-1535
Keywords : Sodium Lactate; HPLC Method; Development; 210nm;
Abstract
A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for rapid assay of 60 % SODIUM LACTATE NaC3H5O3. Isocratic elution at a flow rate of 1.2ml /min was employed on a symmetry phenomenex hypersil L17 (300mm x 7.80 mm, 8 m- rezex RHM - monosaccharide H+) column at ambient temperature. The mobile phase consisted of 0.1M Sulphuric acid. The UV detection wavelength was at 210 nm. Linearity was observed in concentration range of 0.001 - 0.005gm/ml. The retention time for 60 % Sodium Lactate was 4.0 min. The method was validated as per the ICH guidelines. The proposed method can be successfully applied for the estimation of 60 % Sodium Lactate.
Other Latest Articles
- Design of High Speed 32 Bit Multiplier Architecture Using Vedic Mathematics and Compressors
- Retinal Blood Vessel Segmentation for Diabetic Retinopathy Using Multilayered Thresholding
- Application of MCNP Perturbation Technique and Difference Method in AP1000
- Optimization of Fracture Behavior of AA6061 Al-Alloy Pipes Using Finite Element Analysis
- Bacteriological Profile of Orthopedic Patients in a Tertiary Care Hospital, Bengaluru
Last modified: 2021-06-30 21:49:27