Bacteriological Profile, Antibiotic Sensitivity Pattern and Detection of ESBL Production in the Isolates of UTI in Tertiary Care Hospital, Davangere, India

Bacteriological profile, Antibiotic sensitivity pattern and detection of ESBL production in the isolates of UTI in tertiary care hospital. Background -Urinary tract infection (UTI) is a very common disease that can affect anyone at any age where the infection rate is higher in women than men. Objective-The aim and objectives of this study were to determine the etiological bacterial pathogens of the UTI and to determine the antibiotic sensitivity pattern of pathogens isolated as well as identify Extended-spectrum -lactamases (ESBL) producers. Methodology-This was a hospital based cross sectional study in which 120 midstream urine samples were collected from April 2015 to June 2015 from clinically suspected UTI patients of various departments. Urine culture was done, using conventional microbiological techniques. Biochemical testing was used to identify the organisms and antibiotic sensitivity was done by the Kirby Bauer disc diffusion method according to standard CLSI guideines. Further ESBLs was detected by double disc synergy and combined disc diffusion test. Results-Out of 120 tested samples, 48 showed growth of pathogens among which the most prevalent were E. coli (29.17 %) followed by Klebsiella (22.92 %). The majority of the isolates were from female (77.08 %). ESBL production was observed in 35.71 % of E. coli strain and 27.27 % of Klebsiella strains. High rates of resistance was found with Ceftazidime (81.58 %), Ceftazidime + Clavulanic acid (76.32 %, Erythromycin (53.33 %), Cefotaxime (53.19 %), Ciprofloxacin (45.83 %), Norfloxacin (43.33 %) among the isolates but Nitrofurantoin (37.93), Gentamycin (22.97 %) and Amikacin (18.75 %) are comparatively sensitive. Conclusion-The study revealed that E. coli was the predominant bacterial pathogen of UTIs. An increasing trend in production of ESBLs among UTI pathogens were noted. Proper knowledge of susceptibility pattern of uropathogens is crucial in order to discourage the indiscriminate use of antibiotics as well as in formulating effective empiric therapy.