Laboratory Studies of Pseudomonas Sp. in Bioremediation of Lubricant Oil Pollution

Wide varieties of microorganisms present in soil are indigenous to remediate the polluted environment. Used engine oil pollution causes a devastating effect on both terrestrial and aquatic environment. The microbes are isolated based on their ability to utilize the used engine oil as sole carbon source and energy. Pseudomonas species isolated from oil contaminated site showed major growth proliferation on used engine oil as sole carbon source. Isolated Pseudomonas Sp. degraded more than 95 % of hydrocarbons present in the used engine oil within the period of 28 days. The growth and pH parameters of the organism were studied between different mediums containing used engine oil as sole carbon source. The percentage degradation of residual oil was evaluated using Gas chromatography (GC) equipped with Flame Ionization Detector. Presence of heavy metals was also detected before and after remediation by Energy dispersion Spectroscopy (EDS) and characterized using Scanning Electron Microscope (SEM). This in-vitro study on remediating used engine oil pollution under stimulated condition can be used as efficient approach on reclaimination and restoration of oil polluted environment by microorganisms.