Production of Bioethanol from Lignocellulosic Biomass by Simultaneous Saccharification and Fermentation

Cellulose degrading microbes such as Bacillus subtilis, Aspergillus niger, and Trichoderma viride were isolated. Lignocellulosic biomasses selected for the production of ethanol by Simultaneous Saccharification and Fermentation were sugarcane bagasse, saw dust, rice chaff. These lignocellulosic biomasses were pretreated by alkaline pre treatment method. Enzymatic Hydrolysis was done by cellulose degrading microbes on the lignocellulosic biomass. Two fermentation microbes were selected which were Saccharomyces ceriviseae and Zymomonas mobilis (MTCC 2428) which was obtained from IMTech, Chandigarh. The distillate obtained from the fermentation broth was analyzed using High Performance Liquid Chromatography.