Structural and Functional Characterization of Hericium erinaceum, Manganese Peroxidase as an Antioxidant against Iron Induced Parkinson's Disease

Hericium erinaceum, (lions mane) is an edible and medicinal mushroom in the tooth fungus group. This has great therapeutic benefits in treating Parkinsons disease mainly because of the stimulation of the Nerve Growth Factor. Most research was focused on the therapeutic effects of the medicinal mushrooms, but little information is available about their antioxidant properties. In this paper, a bioinformatics and molecular modeling approach was adopted to explore properties and structure of Hericium erinaceum antioxidant proteins. The antioxidant proteins studied for the neuroprotective effect by free radical scavenging are manganese peroxidase 1 (MnP1) and manganese peroxidase 2 (MnP2). Physico-chemical characterization interprets properties such as pI, EC, AI, GRAVY and instability index and provides data about these proteins and their properties. Prediction of motifs, patterns, disulfide bridges and secondary structure were performed for functional characterization. Three dimensional structures of these proteins were not available as yet in PDB. Therefore, homology models for these antioxidant proteins were developed. The modeling of the three dimensional structure of these proteins shows that models generated by Swiss model were more acceptable in comparison to that generated by (PS) 2-V2, Phyre2. The quality and reliability of the models were checked using Ramachandran Plot calculation and by using ERRAT. These structures will provide a good foundation for functional analysis of experimentally derived crystal structures