Production and Immobilization of extracellular Lipase from Pseudomonas aeruginosa.

Lipases (Triacylglycerol acylhydrolases, EC 3.1.1.3) catalyzes the enzymatic hydrolysis of triacylglycerol into diacylglycerol & free fatty acids. Lipases act as a key enzyme that is used in various industries like Detergent, Food, Leather, Textiles, Pharmaceuticals, etc. The present study investigates the potentials of Pseudomonas spp. isolated from oil contaminated soil samples in the production and immobilization of Lipase enzyme. Submerged fermentation of SP45 (Pseudomonas aeruginosa) was performed for the production of Lipase. Extracellular SP45 Lipase was then partially purified by ammonium sulphate precipitation followed by dialysis. SP45 Lipase precipitated with 50 % saturated solution of ammonium sulphate showed optimum Lipase activity of about 56.0433U/ml and it was increased upto 101.2400U/ml after dialysis. Purified SP45 Lipase was then immobilized using physical adsorption and microencapsulation methods. SP45 Lipase immobilized in sodium alginate beads showed greater enzymatic activity when compared with SP45 Lipase immobilized with ion exchange resins.