Isolation and Culture Conditions Optimization For PHB Production by Pseudochrobactrum asaccharolyticum

The extensive uses of petroleum based synthetic plastics have resulted in losses of natural resources and accumulation in the environment. It has been a major cause of global environmental concern. Poly--hydroxybutyrate is a potent replacement to petroleum based synthetic plastic, because has mechanical properties similar to polypropylene and completely biodegradable too. In current piece of work, an attempt was made to isolate potent PHB producing bacteria from different root nodule, soil and water samples. Sudan Black B was used for primary screening of bacterial isolates for PHB production. Presence of functional groups in extracted PHB was confirmed with the help of FITR analysis. Culture media conditions having incubation time period 72 h, temperature 30C, pH 7, glycerol as carbon source, ammonium sulphate as nitrogen source and CN ratio as 161 were found to be supportive for maximum PHB production by isolate M7 (2). Pseudochrobactrum asaccharlyticum is first time reported as a PHB producing strain.