Isolation and Pure Culturing of Elite Strains of Blue Green Micro Algal forms for Biofertilizer as initial Cultures from Swami Ramanand Teerth Marathwada University Nanded, Maharashtra

Algae are photosynthetic thallophytes or lower plants which grow in water or on soil saturated with water. Blue green algae are distributed worldwide to enhance the fertility of many agricultural ecosystems. Blue green algae are a group of microalgae that can fix the atmospheric nitrogen. Isolation of these cyanobacteria from natural sources in pure form is essential step for their efficient use as biofertilizer. In present investigation three species of blue green microalgal forms are isolated from natural fresh water and soil habitats of S. R. T. M. U. Nanded. The isolation of pure cultures were done by selecting a single colony from mix cultures grown on selected media like BG-11, bold basal media, ASN III media as different BGA strains can grow on different media. The same media in solid form is used for further purification and sub culturing. Pure culturing was done by adding streptomycin as an antibiotic and fluconazole as an antifungal agent. The pure cultures were then transferred in solid and liquid media for further studies. Special observations were made on their growing pattern on solid as well as in liquid culture.