Ascorbic Acid (Antioxidant) in Aegle Marmelos and Moringa Oleifera and Effect of Growth Regulators and Salts on it in Vitro

Aegle marmelos and Moringa oleifera are two medicinally important plant species. Unorganized tissues of these plants were established on MS medium supplemented with 1.0mg/L BAP+2.0mg/L 2, 4-D and 1.5mg/L BAP +1.5mg/L 2, 4-D respectively (standardized MS medium). Parts of established tissues were transferred to standardized (Sd) MS medium fed with various concentrations (1, 2, 3 mg/L) of growth regulators ( IAA, NAA) and ( 10, 20, 30mg/L) salts ( NaCl, KCl) separately. Tissues at the maximum GI (in all samples) were harvested, dried, powdered and analyzed for estimation of ascorbic acid. Maximum amount of ascorbic acid was calculated in callus fed with 2mg/L IAA, NAA and 10 mg/L NaCl and KCl in Aegle marmelos as well as Moringa oleifera.