Relations between Catharanthine Content Enhancement with the Other Associated Secondary Metabolites in Catharanthus Roseus Cell Culture that Treated Tryptophan

This research was part of a strategy to improve the content of catharanthine. One strategy used was the addition of tryptophan as a precursor. Increased content of catharanthine was essential in in vitro culture in order to keep the catharanthine content at least equal to those grown in nature (in vivo). The research was conducted in the laboratory using MS medium with tryptophan 50-250 mg/L. Results of the determination of secondary metabolites at C. roseus cell aggregates showed that the IAAcontent has optimum with 150 mg/L tryptophan treatment. Tryptophan treatment could increase the content of IAA. Tryptamine content in C. roseus aggregate cells increased with increasing concentrations of tryptophan treatment. Vindoline content in C. roseus aggregate cells after 14 days culture decreased by treatment with 100 to 200 mg/L tryptophan treatment. The increase occurred in E (250 mg/L) of 9.71 0.20 g/g dw in Erlenmeyer and C (150 mg/L) of 11.83 1.01 g/g dw in the bioreactor. Catharanthine content of C. roseus aggregate cells after 14 days of culture was increasing and has optimum content in treatment C (150 mg/L) that was equal to 50.96 g/g dw. The content of vinblastine in C. roseus aggregates cells until day 14 day culture is not detected. But after day 21 of culture vinblastine content detected and has increased as a result of tryptophan precursor treatment. The relationship between other secondary metabolites with catharanhtine content there are 2 correlation. The negative correlation or antagonist that was vindoline. The positive correlation or synergistic ie tryptamine and IAA, vinblastine after being treated tryptophan. This research was part of a strategy to improve the content of catharanthine. One strategy used was the addition of tryptophan as a precursor. Increased content of catharanthine was essential in in vitro culture in order to keep the catharanthine content at least equal to those grown in nature (in vivo). The research was conducted in the laboratory using MS medium with tryptophan 50-250 mg/L. Results of the determination of secondary metabolites at C. roseus cell aggregates showed that the IAAcontent has optimum with 150 mg/L tryptophan treatment. Tryptophan treatment could increase the content of IAA. Tryptamine content in C. roseus aggregate cells increased with increasing concentrations of tryptophan treatment. Vindoline content in C. roseus aggregate cells after 14 days culture decreased by treatment with 100 to 200 mg/L tryptophan treatment. The increase occurred in E (250 mg/L) of 9.71 0.20 g/g dw in Erlenmeyer and C (150 mg/L) of 11.83 1.01 g/g dw in the bioreactor. Catharanthine content of C. roseus aggregate cells after 14 days of culture was increasing and has optimum content in treatment C (150 mg/L) that was equal to 50.96 g/g dw. The content of vinblastine in C. roseus aggregates cells until day 14 day culture is not detected. But after day 21 of culture vinblastine content detected and has increased as a result of tryptophan precursor treatment. The relationship between other secondary metabolites with catharanhtine content there are 2 correlation. The negative correlation or antagonist that was vindoline. The positive correlation or synergistic ie tryptamine and IAA, vinblastine after being treated tryptophan.