Hemocyte and Biochemical Changes of Antheraea mylitta D. Infected with Antheraea mylitta Cytoplasmic Polyhedrosis Virus (AmCPV)

The hemocyte and biochemical changes in Antheraea mylitta after per oral inoculation of Cytoplasmic Polyhedrosis Virus (AmCPV) was observed. The total haemocyte count (THC) increased gradually from day 1 (13650/mm3) to 8 (16625/mm3) in uninoculated silkworms kept as control. In AmCPV treated silkworm larvae the THC increased up to day 2 (12700 - 14480/mm3) of inoculation. Thereafter, a decrease was noticed from day 3 - 8 (14350 - 3812/mm3). The differential haemocyte count (DHC) was different in inoculated silkworms then the control lot of silkworms. In the haemolymph of control larvae the prohaemocyte, plasmatocytes and granulocytes were more in number whereas oenocytoids were less in number. The number of degenerated cells increased among treated lot up to 8th days of post inoculation. The total protein content in the haemolymph of treated larvae during the first and second day was similar to that of control. On third day, the protein content increased among treated larvae. But from fourth day onwards, the protein content decreased (20.25 mg/ml) against control (33.73 mg/ml). The total carbohydrate content gradually increased during fifth instar in control lot larvae, whereas, it steadily increased up to 4th day and then showed a down ward trend from 5th day treated larvae. The total lipid content in the haemolymph decreased among treated larvae day 2, whereas in control larvae, an increasing trend was noticed.