Characteristics of New Primary Oral Cancer (Sp-C1) Cell and Antitumor Activity of Oligonucleotide p27Kip1 Sense on a Sp-C1 Cell

New primary oral cancer (Sp-C1) cell has some characteristics of cell growth, expression of anti-apoptosis protein and positive regulatory protein of cell cycle. Characteristics of Sp-C1 cell is very important to facilitate the researchers using an in vitro model of oral cancer cells. In the present study, p27Kip1 sense (p27 S), p27Kip1 antisense (p27AS) and p27Kip1 neo (p27 neo) were transfected into Sp-C1 cells. The aim of study was to introduce the new primary oral cancer cell with their characteristics and to examine the antitumor activity of oligonucleotide p27 S on Sp-C1 cell in vitro. Isolasion of Sp-C1 cell was derived from cervical lymph node tissue of tongue cancer patient. As a final step of isolation, new fibroblast and primary oral cancer cells were cultured and stocked. The efficiency of p27 S and p27 AS transfection in cell growth inhibition test was confirmed by MTT [3- (4, 5-dimethythiazol-2-yl) -2, 5-diphenyltetrazolium bromide] assay. Colorimetric assay caspase-3 and caspase-9 were carried-out to know the apoptosis induction. The efficiency of oligonucleotide p27 transfection at protein level was confirmed by Western blotting analysis. Results of study revealed characteristics of Sp-C1 was relative fast in cell growth and positive expression of Skp2, -tubulin, CDK-2, cyclin-E, and maspin protein. Furthermore, Sp-C1 cell growth was markedly suppressed by p27 S. Also, p27 S-treated cell induced apoptosis characterized by an increase proteolytic activities of caspase-3 and -9. Up-regulation of p27Kip1 protein was detected in Sp-C1 p27 S. In conclusion, Sp-C1 cells had a certain characteristics and appropriate for an in vitro study model of oral cancer. p27 S had a strong antitumor activity on Sp-C1 cell. Targeting this molecule could represent a promising new therapeutic approach for this type of cancer.