Isolation and Identification of the Pathogens Causing Cycas panzhihuaensis Stem Rot Disease

The objective of the research is to explore the pathogenesis of the C. panzhihuaensis stem rot disease as well as to find out the prevention and treatment of it. Sick tissue with typical symptoms of the stem rot disease was collected in C. panzhihuaensis National Nature Reserve. Pathogens were isolated using the routine tissue isolation method, and the pathogenicity study was carried out. In addition, routine morphological identification and rDNA ITS sequence analysis were also applied to the strains with pathogenicity. The results showed that among the four strains isolated from the ill-healthy tissue, the infection rate of Cym001 reached 76.3 % 15 days after re-inoculation. Hence, Cym001 was determined to be the pathogen causing the C. panzhihuaensis stem rot disease and was then identified to be Fusarium solani. The present research provides a theoretical basis for the prevention and control of the C. panzhihuaensis stem rot disease.