Activated Sludge: Decolorization and Degradation of Textile Dye Reactive Orange HE2R

At lab scale develop an activated sludge and decolorize three tested textile dyes Reactive Red 198, Reactive Orange HE2R and Navy Blue HE2R 13, 17 and 8 % respectively within 120 h. In presence of Glucose (51 %) and yeast extract (98 %) decolorization of Reactive Orange HE2R dye observed within 24 h. The significantly involved laccase and NADH-DCIP reductase in dye decolorization. The analytical studies like UV-Vis spectroscopy, FTIR and HPLC confirmed the degradation of Reactive Orange HE2R. GC-MS data has been proposed the probable dye degraded pathway and metabolites formed identified as 2-amino butane 4-sulpho aniline and sulphoaniline.