Effect of rs2241766Tgreater thanG polymorphism in Adiponectin Gene on Circulating Adiponectin Levels among Iraqi Women with PCOS

BACKGROUND Polycystic ovary syndrome (PCOS) is a most common endocrine /metabolic disorder of women in their reproductive ages. Although PCOS is complex, but there is strong evidence that candidate genes on insulin action have been proposed to show a vital role in its etiology. AIM The current research was designed to study the effect of (rs2241766Tgreater thanG) in adiponectin gene with PCOS, and their impact on the level of serum adiponectin and insulin resistance (HOMA-IR) in Iraqi women with PCOS. METHODS The case control design study was performed on 120 women with and without PCOS. Phenotypic analysis included the estimation of fasting levels of, blood glucose, and lipid profile by spectrophotometric methods, while insulin, and adiponectin concentrations were determined by ELISA. Genotyping of the selected SNP (rs2241766Tgreater thanG) in the adiponectin gene was carried out by (PCR-RFLP) technique. RESULTS After controlling for the age, body mass index, significant difference in Waist circumferences, fasting insulin, HOMA-IR, and serum adiponectin levels were found in POCS group with respects to those of a healthy control group. Genotyping and alleles results of the selected SNP (rs2241766Tgreater thanG) were not associated with incident of PCOS. However, women with PCOS with TG and GG genotype had higher insulin levels, and HOMA-IR than carriers of the TT genotype. CONCLUSIONS The current study point out that the selected SNP (rs2241766Tgreater thanG) in adiponectin gene is not related to PCOS in Iraqi women. Nevertheless, the selected polymorphism was found to be associated with insulin levels, and HOMA-IR among Iraqi women with PCOS, and to impact adiponectin secretion. Further research with a greater sample size, and more SNPs in adiponectin gene are wanting to confirm these results.