In Vitro Multiplication of Ceropegia Bulbosa VAR. Lushii Roxb

Ceropegia bulbosa var bulbosa Roxb is member of family Asclepiadaceae which was described by William Roxburgh. It is widely distributed, growing sporadically almost throughout India in red laterite soil. The leaves are subsessile to petiolate while Ceropegia bulbosa var. bulbosa has orbicular to ovate. It cures the disorder Urolithiasis which is the development of stones in the urinary tract. This may lead to pain and bleeding. It is considered as the third most common affliction of the urinary tract. In most of the types of stones that are formed, the most frequent are calcium oxalate. As per clinical and epidemiological studies, calcium oxalate followed by calcium phosphate is the most commonly encountered crystalline components found in urolithiasis. During present piece of work nodal explants from mature plant were surface sterilized and aseptically cultured on MS medium supplemented with various concentrations (0.5, 1.0 and 3.0 mg l-1) of cytokinins (BAP and Kn) and auxins (IAA, NAA and 2, 4-D) alone and in various combinations under controlled condition. Successful regeneration was achieved by this method.