Synthesis and In Vitro Evaluation of Anticancer activity of Mannich Bases of Benzimidazole Derivatives

Benzimidazole moiety is an important class of heterocycles used for synthesis of medicinal compounds. Mannich bases of Benzimidazole were synthesized. The structures were identified by determining their melting points, TLC, IR spectral analysis and 1HNMR spectrum. Anticancer activity was performed in vitrofor screening of synthesised compounds by Sulfordamine B (SRB) assay. Cytotoxicity of compounds was evaluated for lung, prostrate, colon and breast tissue. Compound VIII a and VIII bshowed 100 % cytotoxicity against colon cancer. The compound VI c showed 89 % cytotoxicity against lung cancer. Against prostate cancer compound VIII a resulted in 73 % cytotoxicity. The compound VIII a and VI a showed 94 %, 93 % cytotoxicity respectively against breast cancer.